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KMID : 0438219750120030733
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Korea University Medical Journal
1975 Volume.12 No. 3 p.733 ~ p.741
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The Effects of γ-Aminobytyric Acid and Other Related Compounds on the Metabolism of Rat Brain Slices
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Abstract
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This experiment was carried out to investigate systematically how 7-aminobutyric acid (GABA) and other related compounds, r-amino-p-hydroxybutyric acid (GABOB) and pyridoxine (B6), effects on metabolism of central nervous system.
120 albino rats were divided into 2 groups, i.e., in vitro and intraventricular injection groups. Each group was subdivided into 6 groups, i.e., control, GABA, GABOB, B6, GABA with B6 and GABOB with BB group.
In vitro groups 5mM GABA or GABOB, and 0. lmg of B6 were added into incubation medium, while in intraventricular injection group 0.2ml of 0.5M GABA or GABOB and 0. lml of B6 (5mg) were injected into lateral ventricle.
The oxygen consumption rate of the slices of cerebral cortex, thalamus, medulla oblongata, cerebellum, and spinal cord in albino rat were determined using the Warburg manometric apparatus supplying 100% oxygen.
The results are summarized as follows:
1. GABA increased the oxygen consumption rate of brain more significantly in the intraventricular injection group than in vitro group.
2. GABOB decreased the oxygen consumption rate of brain. A significant decrease were s3en at medulla oblongata in vitro group and that of cerebral cortex and cerebellum in the intraventricular injection group.
3. Pyridoxine decreased the oxygen consumption rate of brain stem in vitro group. On the other hand the oxygen consumption rate of cerebral cortex and cerebellum were increased except medulla oblongata in the intraventricular injection group.
4. GABA with B6 decreased the oxygen consumption rate of cerebral cortex only in vitro group, whereas increased that in the intraventricular injection group.
5. GABOB with B, decreased the oxygen consumption rate of cerebral cortex, cerebellum and thalamus in vitro group. On the other hand the oxygen consumption rate of cerebral cortex increased in the intraventricular injection group.
According to results of the above findings, it may be speculated that GABA may be utilized as substrate, while GABOB can not be oxydized in the brain tissue level. However, the effect of B, on the above changes are different in vivo from that in vitro experiment.
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